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Literature summary extracted from

  • Font, J.; Benito, A.; Torrent, J.; Lange, R.; Ribo, M.; Vilanova, M.
    Pressure- and temperature-induced unfolding studies: thermodynamics of core hydrophobicity and packing of ribonuclease A (2006), Biol. Chem., 387, 285-296.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
4.6.1.18 I106A detailed study on thermodynamic parameters Bos taurus
4.6.1.18 I107A detailed study on thermodynamic parameters Bos taurus
4.6.1.18 I81A detailed study on thermodynamic parameters Bos taurus
4.6.1.18 additional information series of mutations in residues V54, V57, I106 and V108 most critical for stability. Detailed study on thermodynamic parameters Bos taurus
4.6.1.18 V108A detailed study on thermodynamic parameters Bos taurus
4.6.1.18 V116A detailed study on thermodynamic parameters Bos taurus
4.6.1.18 V118A detailed study on thermodynamic parameters Bos taurus
4.6.1.18 V47A detailed study on thermodynamic parameters Bos taurus
4.6.1.18 V54A detailed study on thermodynamic parameters Bos taurus
4.6.1.18 V57A detailed study on thermodynamic parameters Bos taurus
4.6.1.18 V63A detailed study on thermodynamic parameters Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
4.6.1.18 Bos taurus P61823
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.6.1.18 pancreas
-
 Bos taurus
-

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.6.1.18 additional information
-
 heat and pressure produce only slightly different energetic changes in the unfolded state of enzyme. Stability differences in mutants can be attributed to both hydrophobic interactions and packing density Bos taurus
4.6.1.18 43.1
-
 melting temperature, mutant I106A Bos taurus
4.6.1.18 43.3
-
 melting temperature, mutant V108A Bos taurus
4.6.1.18 45
-
 melting temperature, mutant V47A Bos taurus
4.6.1.18 47.7
-
 melting temperature, mutant I107A Bos taurus
4.6.1.18 47.7
-
 melting temperature, mutant I81A Bos taurus
4.6.1.18 48.7
-
 melting temperature, mutant V57A Bos taurus
4.6.1.18 49.8
-
 melting temperature, mutant V54A Bos taurus
4.6.1.18 52.5
-
 melting temperature, mutant V118A Bos taurus
4.6.1.18 54.8
-
 melting temperature, mutant V116A Bos taurus
4.6.1.18 58
-
 melting temperature, wild-type Bos taurus
4.6.1.18 58.1
-
 melting temperature, mutant V63A Bos taurus